The most common way to manufacture oligonucleotides of various types like antisense, small interfering RNA (siRNA), and aptamers is by means of the phosphoramidite route. This solid-state process links individual nucleotides together in a step-by-step process consisting of detritylation, coupling, capping, and oxidation/thiolation.
The linkage of the various nucleotides to each other is achieved via the C-3’ and C-5’ hydroxy function of the furanoses of the individual nucleosides. One of the most efficient protecting groups widely used to protect the C-5’ hydroxy function is 4,4’-dimethyoxytrityl (DMT). The subsequent removal of this group in the detritylation step is achieved by dichloroacetic acid (DCA).
Two manufacturing sites for a safe supply of large quantities
CABB has two independent large-scale manufacturing sites for DeTrityl® Ultrapure in Germany and Switzerland. This provides a significant benefit for customers from a GMP and risk mitigation perspective. DeTrityl® Ultrapure is manufactured with state-of-the-art technologies to ensure consistency, quality and reliability.